Bacterial Endotoxin Tests

Bacterial Endotoxin Tests

We can briefly describe this word as a bacterial component. Not only the peptidoglycan layer, but also the outer membrane layer is located on the cell walls of gram-negative bacterial species. Endotoxin is a component of the outer membrane we have mentioned. Structurally mixed lipopolysaccharide structure. Also, the core comprises a plosaccharide chain, an o-specific polysaccharide side chain and a component called lipid.

For those of interest, we would like to note that the size of the endotoxin is in the range of 10 kDa to 1.000 kDa. Because it is a structural bacterial component, it occurs when any bacterium loses its normal structure. If we say otherwise, it is a fragmented bacteria structure.

What is a Bacterial Endotoxin Test?

When we call the bacterial endotoxin (LAL) test, it means the formation of toxic structures formed on the cell walls of gram negative bacteria on the product. Attention should be paid to various medical devices that are in contact with our body, as well as to drugs and dialysis waters. In addition, the number of samples to be studied in accordance with the number of batches may vary during production.

If we briefly summarize endotoxins, this is the last weapon type that can be used just before the death of gram negative bacteria. Endotoxin contamination is often used in important areas of 3. These include dialysis treatment, preparation or packaging of medical devices, drugs intended for injection and production of biological products.

Dialysis Treatment

We would like to state that the water to be used when dialysis treatment is very important. Maximum care must be taken to prevent damage to the immune system in sick persons. It is essential that this care is vital. In order to meet this need, it is possible to reduce the amount of endotoxin by reducing or eliminating the values ​​indicated in the pharmacopoeia.

Extreme caution is necessary as water or any product in contact with the patient is related to endotoxin contamination. Contact with water can be transmitted during the washing of instruments. Even if you try to prevent it, you cannot prevent the transmission of the disease. Because the type of endotoxin bacteria can be transmitted at any time. Some precautions can be taken to prevent the contamination of the bacteria, but unfortunately you cannot eliminate the risk of contamination, no matter how many precautions are taken.

Recently, the most common negative enteric bacilli are inadequate tissue perfusion, disseminated intravascular coagulation and vascular damage. The occurrence of endotoxin in human blood is called endotoxemia, which can lead to septic shock later.

In addition, endotoxin waste treatment is carried out in pharmaceutical boxes. It is very important to do this procedure in order not to infect the person using the drug. A temperature higher than 300 C is required to ensure the destruction of the LPS. Decrease in endotoxin is related to factors such as warming time and temperature. In some specially prepared depyrogenation furnaces used for this purpose, endotoxin levels are reduced by 250 times when glass ampoules and also syringes are kept in 30 C for a period such as 1000 minutes.

The Limulus Amebocyte Lysate test variant is known to be one of the most sensitive tests used to determine the presence or absence of endotoxin. Blood from horseshoe crab is used during this test. Very few LPS or any amplification causes the coagulation of the limulus lysate. Of course, there are also different methods for performing bacterial endotoxin tests. Each of these varieties can be administered to patients by different methods.